HIV RESERVOIR CHARACTERIZATION WORKSHOP

25-27 September
Ghent, Belgium

Program

Day 1 - Wednesday Day 2 - Thursday
Day 3 - Friday
08.30-09.00
HIV Cure Symposium Registration Registration
09.00-10.30
Practical session on dPCR
platforms (Group 2)

How to assess the size of the
viral reservoir by measuring
cell-associated HIV RNA
(Group 1)
Practical sessions
- HIV-PULSE and proviral sequence analyses
- HIV-Flow and STIP-Seq
- MDA and Integration site analysis
- dPCR Data analysis challenges and compensation matrix


10.30-11.30
Assessing the HIV reservoir by near
full-length proviral sequencing
methods
Practical sessions
- HIV-PULSE and proviral sequence analyses
- HIV-Flow and STIP-Seq
- MDA and Integration site analysis
- dPCR Data analysis challenges and compensation matrix
12.15-13.15
LunchLunch
13.15-14.45
Determination of the integration
site of the HIV provirus

Combining multiple assays to
assess the HIV- reservoir: HIV-Flow
and STIP-Seq

Practical sessions
- HIV-PULSE and proviral sequence analyses
- HIV-Flow and STIP-Seq
- MDA and Integration site analysis
- dPCR Data analysis challenges and compensation matrix
14.45-15.00
RegistrationBreak
15.00-16.00Description of dPCR-based
methods
Practical sessions
- HIV-PULSE and proviral sequence analyses
- HIV-Flow and STIP-Seq
- MDA and Integration site analysis
- dPCR Data analysis challenges and compensation matrix


16.00-17.00How to assess the size of the viral
reservoir by measuring total and
intact HIV- DNA
Practical sessions
- HIV-PULSE and proviral sequence analyses
- HIV-Flow and STIP-Seq
- MDA and Integration site analysis
- dPCR Data analysis challenges and compensation matrix
17.00-18.30Practical session on dpcr platforms (Group 1)
19.00-22.00
Dinner