HIV RESERVOIR CHARACTERIZATION WORKSHOP 2022

Welcome

The HIV Cure Research Center Ghent is very pleased to invite you to the 6th HIV Reservoir Characterization Workshop that will be held in the beautiful city of Ghent, Flanders, Belgium on September 14-16, 2022.

The HIV Reservoir Characterization workshop is for researchers that are interested in low level RNA or DNA quantification by digital PCR and immune monitoring assays especially in the field of HIV research.

This workshop will provide you with a thorough understanding of HIV quantification tools including PCR-based and cell-based functional assays, which are of great importance in therapy monitoring for the development of HIV cure strategies. The participants will receive an intensive training on PCR, qPCR, digital and digital droplet PCR methods with a comprehensive overview of clinical use of these methods and clinical significance of various HIV markers. Novel aspects of HIV persistence such as clonal expansion and integration site will also be discussed and thorough overview of assays to monitor them will be presented. Given that the host’s immune system is an indispensable component of an effective cure strategy, in addition to virological assays, the workshop will present an overview of immunological assays that are commonly used to assess the potency of both the cellular and humoral immune system. The workshop will conclude with comprehensive hands-on sessions that will include statistical data analysis training sessions.

Program

September, 14-16, 2022

Time Zone: Central European Summer Time (CEST)

 DAY 1 - Sept 14

Virology part 1
DAY 2 - Sept 15

Virology part 2 and Immunology
DAY 3 - Sept 16

Hands-on training
08.00-09.00Registration and breakfastRegistration and breakfastRegistration and breakfast
09.00-10.30Clinical studies to evaluate
markers of HIV reservoir and how to set up a clinical trial

Prof. Dr. Linos Vandekerckhove
Assessing the HIV-1 reservoir
by near full-length sequencing methods


Ir. Laurens Lambrechts, PhD
Hands-on training:
-HIV Flow
-UMI
-IPDA
10.30-11.30Description of PCR based methods: PCR, QPCR,
Digital PCR, digital droplet PCR

dr. Sofie Rutsaert
Determination of the site of integration of the HIV-1 provirus


Ir. Tine Struyve, PhD
Hands-on training:
-HIV Flow
-UMI
-IPDA
11.30-12.15How to assess the size of the viral reservoir by measuring total and
intact HIV DNA

Mareva Delporte, PhD
Combining multiple assays to assess the HIV- reservoir:
MIP-Seq and STIP-seq

dr. Marion Pardons
Hands-on training:
-HIV Flow
-UMI
-IPDA
12.15-13.15LunchLunchLunch
13.15-14.00How to assess the size of the viral reservoir by measuring
cell associated RNA

Willem van Snippenberg, PhD
In vitro and ex vivo HIV killing assays



dr. Wojciech Witkowski
Analytical set-up



14.00-14.45How to assess the size of the
viral reservoir by FISH-Flow

dr. Shringar Rao
An immunological workflow for HIV clinical study samples

Dr. Jozefien De Clercq
Presentations of the
practical exercise

14.45-15.00BreakBreakClosing Remarks
and feedback
15.00-15.45The size of the translational
competent viral reservoir by
HIV-Flow

dr. Marion Pardons
Analysis of T cell metabolism via Seahorse technology


dr. Bram Van Den Eeckhout
15.45-16.30Quantification of the Productive
HIV-1 Reservoir by VIP-SPOT

Maria Carmen Puertas, PhD
Immunological assays to measure
HIV-specific T cell responses

Dr. Ane Ogbe
16.30-17.00BreakBreak
17.00-18:30Practical exercises on how to assess
the viral reservoir in cure studies

Prof. Dr. Linos Vandekerckhove
Dr. Jozefien De Clercq
Dr. Nele De Langhe
Practical exercises on how to assess the viral reservoir in cure studies

Prof. Dr. Linos Vandekerckhove
Dr. Jozefien De Clercq
Dr. Nele De Langhe
19.30-22.00Diner

Registration

Venue

The three day workshop will take place in the MRB 2 building at the University Hospital (UZ) Campus in Ghent, Belgium.

How to get there?

Tram 4 drives every 7,5 minutes between Gent-Sint-Pieters station and the heart of UZ Gent. The platform is located near the parking building.

Buses 5 and 8 drive every 15 minutes from the city center (South) and the Heuvelpoort to the edge of the campus, within walking distance of the clinic buildings.

Tram 2 and buses 47, 65 and 67 also have stops around the UZ campus.

Organizing Committee

Prof. Dr. Linos Vandekerckhove – Head of the HIV Cure Research Center – Ghent University Belgium

Prof. Dr. Sarah Gerlo – PI HIV Cure Research Center – Ghent University – Belgium

Karen Vervisch – Lab Manager – Ghent University Hospital – Belgium

Contact

Karen Vervisch
Karen.vervisch@uzgent.be
+32 (0) 9 332 94 25

Sponsors

If your company is interested in sponsoring the HIV Reservoir Characterization workshop, please contact karen.vervisch@uzgent.be