Arrayed CRISPR-interference screening platform
Arrayed CRISPR-interference screening platform
Platform to perform perturbation of individual genes in an arrayed setup using CRISPR-interference technology. Different model cell lines that were stably transduced with the CRISPR-interference machinery. High-throughput shallow RNA-sequencing readout to establish the molecular phenotype for each perturbation.
What will you obtain:
Knock-down for dozens (up to 100) genes in a single experiment with matching gene expression data for each knock-down condition. Additional readouts are possible (including cell proliferation, apoptosis, …).
Why should someone choose this asset:
Therapeutic or disease target identification, molecular characterization of genes with unknown function (eg. long non-coding RNAs), characterization of gene function in various cell states.
Unique selling points:
- Several validated CRISPR-interference model cell lines
- Comprehensive readout through RNA-seq for molecular phenotyping
Can the asset be used on a broader setting (beyond HIV)?:
Yes, any disease for which model cell lines are available. Additional model cell lines can be generated on demand
Type of collaboration possible:
☒ | Fee for service |
Contact:
Pieter Mestdagh
pieter.mestdagh@ugent.be
WERELDWIJD LEVEN 37 MILJOEN MENSEN MET HIV. STEUN HET FONDS HIV ONTRAFELEN en Help hen.
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Of via overschrijving:
Universiteitsfonds UGent - IBAN BE26 3900 9658 0329
- Met duidelijke vermelding: "Fonds HIV"
- Giften zijn voor 45% fiscaal aftrekbaar vanaf 40 euro op jaarbasis.
- Meer info over hoe je het HIV onderzoek kan steunen.