| Day 1 - Wednesday | Day 2 - Thursday | Day 3 - Friday | |
|---|---|---|---|
| 08.30-09.00 | Registration | Registration | Registration |
| 09.00-10.30 | Clinical studies to evaluate markers of HIV reservoir and how to set up a clinical trial | Assessing the HIV reservoir: near full-length proviral sequencing methods and integration site analysis | Integrating omics and viral reservoir data |
| 10.30-11.30 | Description of dPCR-based methods | Assessing the translation-competent HIV reservoir: HIV-Flow | Integrating omics and viral reservoir data |
| 11.30-12.15 | How to assess the size of the viral reservoir by measuring total and intact HIV DNA | Combining multiple assays to assess the HIV- reservoir: MIP-seq and STIP-Seq | Meet the expert: experimental setup and data analysis: HIV-PULSE, IPDA, HIV-Flow, Data integration, FLIPS and ISLA |
| 12.15-13.15 | Lunch | Lunch | Lunch |
| 13.15-14.45 | Practical session on dPCR platforms | Practical sessions on: -HIV-PULSE -HIV-Flow - STIP-Seq -Limiting dilution assays | Presentations of the practical exercise |
| 14.45-15.00 | Break | Break | Closing Remarks and feedback |
| 15.00-16.00 | How to assess the size of the viral reservoir by measuring cell- associated HIV RNA | Practical sessions on: -HIV-PULSE -HIV-Flow - STIP-Seq -Limiting dilution assays | |
| 16.00-16.45 | Data analysis challenges and compensation matrix | Practical exercises on how to assess the viral reservoir in cure studies | |
| 16.45-17.00 | Break | ||
| 17.00-18.30 | Practical exercises on how to assess the viral reservoir in cure studies | ||
| 19.00-22.00 | Diner |